文档介绍：: .
中国烟草学报-(alpha-D-glucosaminyl)-1D-myo-inositol 、茄啶（Solanidine ）、贝磷地尔（belfosdil ） 、 硅 雄 ***
（Silandrone）。【结论】青枯病烟田和健康烟田土壤代谢物组成上存在较大差异，筛选出 8 种代谢标志物可为判别
土壤感烟草青枯病状况提供一定参考。
关键词 烟草青枯病；根围土壤；非靶向代谢组学；代谢标志物
Analysis of metabolic markers in soil with tobacco bacterial wilt based on
metabolomics
WANG Rui, TAN Jun, FAN Jun, XIANG Bikun
Enshi Tobacco Company of Hubei Province, , Shizhou Road, Enshi, 445000, Hubei, PR China;
Abstract [Objective] This study aims to analyze difference of soil metabolites composition between healthy tobacco field
and field with bacterial wilt, and screen metabolic markers. [Method] By taking the rhizosphere soil of diseased plants and
healthy plants as the research object and using LC-MS / MS analyzing technology of nontargeted metabonomics, the
metabolites composition in rhizosphere soil in three periods was studied. [Result] (1) There were 115, 1 59 and 105
metabolites significantly different between healthy tobacco field and field with bacterial wilt at non -onset, middle onset and
late onset stages, respectively. (2) There were 63 common differential metabolites in the three stages, including 49 p ositive
and 14 negative metabolites. The 49 positive metabolites could b be clustered into two groups and seven subgroups. (3) A
total of 8 metabolic markers were screened, including cyclopamine, maltotetraose, Tricine, khelin, 6 - (alpha-d-
glucosaminyl) - 1D myo inositol, solanidine, belfosdil and silandrone. [Conclusion] There were significant different in the
composition of rhizosphere soil metabolites between healthy and diseased tobacco plants. The 8 metabolic markers screened
out could provide support for the identification of soil infection with tobacco