代谢组学研究.ppt

文档介绍：代谢组学研究-?—1Protein—>>1Metabolite1代谢组学的含义metabolomics着重研究的是单个细胞或细胞类型中所有的小分子成分和波动规律,或许可以叫做细胞代谢组学metabonomics则是定量研究有机体对由病理生理刺激或遗传变异引起的,与时间相关的多参数代谢应答代谢组学的发展198219831984198919992000200120022004VanDeGreef:publicationofMSforurineprofilingSadler,BuckinghamandNicholson:Firstpublicationon1H-NMRofbloodandplasmaNicholson,etal.:ponentanalysisofspectradatafromraturineNicholsonandWilson:NMRspectroscopyofbiofluidsNicholson:DefinitionofMetabonomicsHaselden,etal.:FirstindependentPharmapublicationofMetabonomicsNicholson,Lindon,andHolmes:PublicationinNatureonMetabonomicsHolmesandAnttiExplanationofstatisticsinMetabonomicsIncreasing#ofpublicationsChallengesofMetabonomicsplexityandDataHandlingEachsamplehas.., poundclasses ponentsEachgroupofsampleshas…, manysampleanalysesarerequiredforstatisticalrelevance acomplexrawdatasetthatneedstobeprocessed parisonMetaboliteprofilingproducesinformationonthebiochemicalpathwayseffectedMonitoringmetabolitesallowsconcurrentorsequentialaffectstobemonitored,(hydrazine)Metabonomics,unliketheother“omics”isnon-invasive代谢组学分析流程样品的采集预处理数据的采集数据的分析及解释Thestrategyforlargescalemetabonomicsresearch样品处理L-LSPMESPE