高效液相色谱高级研修班PPT2.pdf

文档介绍：How Can Temperature Help Overview概要
温度可怎样提供帮助
Adam Woodhouse, Agilent UK,
edited by An Rong, Agilent China,
•Affect of Temperature on Pressure
温度对压力的影响
•Speeding It Up 加速
•Thermal Stability 热稳定性
•Separation Robustness 分离稳定性/耐受性
•UV Sensitivity 紫外灵敏度
•LC/MS Sensitivity 液质联用灵敏度
HPLC MasterClass 2007 Group/Presentation Title
Agilent Restricted
Page 1 安捷伦2006液相色谱技术高级研修班 Month ##, 200X Page 2
Pressure Equation Viscosity Plots
压力公式粘度曲线
ACN/water
Viscosity Specific permeability 乙腈/水
粘度比渗透性 20

25
0 2 2
P = ηFL/K π r dp 30
35

V i sc o s ty 40

Flow rate 45
Column radius
流速 50
柱半径 0 50 100 150
Column length Particle diameter 55
%ACN
柱长粒径 60
Page 3 Page 4
Pressure Equation压力公式 Viscosity Plots
粘度曲线
ΔP = ηFL/K0 π r2dp2
Methanol/water
Consider a 50 x Column with particles at 1 ml/min
x 色谱柱在1 ml/min流速下甲醇/水
20
25
ΔP = Viscosity (cP) x 139
30
35
At 10% Acetonitrile 10%乙腈时 40
Vi s c o i ty
45
o
20 C ΔP = x 139 = 156 Bar 50
o 0 50 100 150
40 C ΔP = x 139 = 105 Bar 55
60oC ΔP = x 139 = 72 Bar %Methanol
60
80oC ΔP = x 139 = 46 Bar
100oC ΔP = x 139 = 33 Bar
Page 5 Page 6
1
Pressure Equation压力公式 Speeding Up加速分析
ΔP = ηFL/K0 π r2dp2
If pressure is red