文档介绍：Increasing Efficiency, Increasing Resolution -
Better Separations
改善分离效率与分离度,获得更好的分离结果
Paul Barton
Masterclass2007 HPLC MasterClass 2007 Group/Presentation Title
Agilent Restricted
Page 1 Page 2 安捷伦2007液相色谱技术高级研修班 Month ##, 200X
Outline概要 Rapid Resolution HT – RRHT Columns in 2007
Sub-2mm particle columns can be used in two ways
oolluummnsns
亚二微米颗粒色谱柱可以根据需要按两种方式选用: 2003 2007
• Fast LC with short, highly efficient columns at conventional andhigh flow
rates用 400 Bar 600600 bbaarr
高效短柱在常规及高流速下进行快速液相色谱分析 Columns/oolluummnnss ((aarrttrrididggees)s)
• High Resolution LC with longer (100mm-150mm) columns and Length: 15, 30, 50 mm Length: 20, 30, 50, 100, 150mm
conventional flow rates
用较长(100mm-150mm)色谱柱在常规流速下进行高分离度液相色谱分析 ID: , ID: , , ,
• Longer (100mm – 150mm) sub-2mm particle columns have much higher
Bonded Phases: 11
system backpressure, but this is less of an issue because we arelooking to Bonded Phases: 5
exploit the efficiency gains, not speed gains – flow remains the same as for Eclipse XDB-C18, C8
Eclipse XDB-C18, C8
5mm or 3mm column SB-C18, C8, CN, Phenyl
SB-C18, C8
较长亚二微米颗粒色谱柱会产生比较高的反压,但此时我们关心的是分离效率 Extend-C18, Rx-sil
而不是速度-流速可以与5mm or 3mm 色谱柱保持相同 Extend-C18 Eclipse Plus C18/C8
Masterclass2007 Masterclass2007
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Effect of Column Length and Particle Size on Resolution Equation分离度公式
Column Efficie