文档介绍：硕士学位论文
磁珠富集法开发西藏沙棘的 SSR 引物及肋果沙棘与西
藏沙棘自然杂交的鉴定
Development of Microsatellite Primers in Hippophae
tibetana by ic Beads Enrichment and the
Confirmation of Natural Hybridization between
and Based on Morphological
and Molecular evidences
许璐
Xu Lu
目录
独创性声明 ................................................................................................................................ I 
摘要 ....................................................................................................................................... II 
Abstract .................................................................................................................................... III 
第一部分磁珠富集法开发西藏沙棘的 SSR 引物 ................................................................. 1 
1. 研究概述 .......................................................................................................................... 1 
DNA 分子标记 ........................................................................................................... 1 
SSR 分子标记的引物开发 ......................................................................................... 6 
引物设计 .................................................................................................................... 9 
应用分子标记研究沙棘属植物的进展及本研究的意义 ...................................... 10 
2. 材料方法 ........................................................................................................................ 13 
材料 .......................................................................................................................... 13 
实验方法 .................................................................................................................. 14 
西藏沙棘微卫星位点的获得和引物的设计 .......................................................... 19 
微卫星引物多态性检测 .........................................................................