文档介绍：184 2010, Vol. 31, No. 13 食品科学※生物工程
杀菌剂抑霉唑人工抗原的合成和鉴定
周映霞 1,2,3,张丽杰 2,潘家荣 2,焦必宁 1,3,*,王磊 2
(,重庆 400716;,北京 100193;
/ 西南大学柑桔研究所,重庆 400712)
摘要:以咪唑乙醇为原料,与 6- 溴己酸乙酯发生取代反应后再经水解反应合成抑霉唑半抗原(6-[1-(2,4- 二***苯基)-
2-(1- 咪唑基)乙氧基]己酸)。产物经薄层层析和 1H-NMR 鉴定后,采用活化酯法分别与牛血清蛋白(BSA)和卵清白蛋
白(OVA)偶联得到免疫原(HIA-BSA)和包被原(HIA-OVA),紫外光谱分析法计算得其偶联比分别为 13:1 和 7:1,初
步说明人工抗原合成成功。通过免疫动物获得效价为 1:64000 的抗体,采用间接竞争 ELISA 法测定抗体的 IC50 值为
,从而进一步证明人工抗原合成成功,所得的抗体可用于 ELISA 检测试剂盒的研制。
关键词: 抑霉唑; 半抗原; 人工抗原; 合成; 鉴定
Synthesis and Identification of Imazalil Antigen
ZHOU Ying-xia1,2,3,ZHANG Li-jie2,PAN Jia-rong2,JIAO Bi-ning1,3,*,WANG Lei2
(1. College of Food Science, Southwest University, Chongqing 400716, China;2. Institute of Agro-Food Sciences and Technology,
Chinese Academy of Agricultural Sciences, Beijing 100193, China.;3. Citrus Research Institute, Chinese Academy of Agricultural
Sciences/Citrus Research Institute, Southwest University, Chongqing 400712, China)
Abstract:In this study, imazalil (6-[1-(2,4-dichloro-phenyl)-2-(1-imidazolyl) ethoxy] hexanoic acid) was synthesized via
substitution reaction between imidazole ethanol and 6-bromo-ethyl followed by hydrolysis reaction to provide a hapten. The
synthesized product was identified by thin-layer chromatography (TLC) and 1H-NMR. The hapten was conjugated with bovine
serum albumin (BSA) and