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Example #13: Testing Precision of Peak Retention Times and Areas of an HPLC
System
The following is an example of an operating procedure for the testing of an HP 1050 Series
HPLC system for precision of peak areas and retention times. This is a proposal and starting
point only and may need adaptation to different HPLC systems. There is no assurance
expressed that the operating procedure will pass a regulatory inspection.
Company Title: Testing precision of Code Revision
peak areas and retention number
times of an HPLC system LHEQ01
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1. Scope
Testing the precision of peak areas and retention times of an HP 1050 Series HPLC system.
2. Purpose
The precision of peak areas and retention times are important characteristics for qualitative
and quantitative measurements in HPLC. This operating procedure provides chromatographic
conditions and key sequences to verify these characteristics of plete HPLC system,
comprising an HP 1050 Series Autosampler, a Quaternary Pump and a Variable Wavelength
Detector.
3. Frequency
The precision should be verified at least once a year or after the repair of one or more
modules.
4. Instrumentation
a) Quaternary HP 1050 Series Pump
b) HP 1050 Series A