文档介绍：雾化吸入布地奈德对肺纤维化大鼠肺组织MMP2、
【摘要】目的探讨布地奈德雾化吸入对博莱霉素(BLM)致肺纤维化大鼠肺组织基质金属蛋白酶2(MMP2)及金属蛋白酶抑制剂2(TIMP2)表达的影响。方法组)、生理盐水空白对照组(NS组)和布地奈德干预组(Bud组)各15只,BLM组和Bud组气管内灌注博莱霉素诱导肺纤维化,NS组在相同条件下灌注生理盐水。气管内灌注药物后,第0~6天Bud组给予布地奈德雾化吸入,其余组在相同条件下给予生理盐水雾化吸入。各组分别于第7、14、28天随机处死5只,收集肺组织作切片、行HE及Masson染色,判断肺组织肺泡炎及肺纤维化程度;行免疫组化染色,检测MMP2和TIMP2在肺组织的表达水平。结果与NS组相比,Bud组第7、14、28天肺组织的肺泡炎和肺纤维化程度明显加重(P<);与BLM组相比,Bud组第7、14天肺组织的肺泡炎程度明显减轻(P<),第7、14、28天肺纤维化程度明显减轻(P<);与NS组相比,BLM组第7、14、28天MMP2和TIMP2表达明显增加(P<);与BLM组相比,Bud组第7、14、28天MMP2和TIMP2表达明显降低(P<)。结论布地奈德雾化吸入可减轻BLM致肺纤维化大鼠的肺泡炎和肺纤维化程度,其干预机制可能为降低肺组织内MMP2及TIMP2的表达,并在一定程度上调整MMP2/TIMP2的比值。
【关键词】布地奈德;肺纤维化;博莱霉素;基质金属蛋白酶2(MMP2);金属蛋白酶抑制剂2(TIMP2)
The Effect of Inhaled Budesonide on the Expression MMP玻 and TIMP玻 in Lung Tissues of Rats onary Fibrosis
Zhang Xiaobo, Fan Xianming, ing, Zhu Chen, the Attached Hospital to Luzhou Medical College, Luzhou, Sichuan Province 646000
ABSTRACT Objective To investigate the effect of inhaled budesonide on the expression of matix metalloproteinases玻玻∕MP玻玻 and tissue inhibitors of metalloproteinases 玻(TIMP玻) in lung tissues of rats ycin-induced pulmonary fibrosis. Methods 45 ) group, the budesonide (Bud) group and the normal solution (NS) group. Intratracheal perfusion of BLM ade to the Bud group and the BLM group to induce pulmonary fibrosis al solution to the NS group in the same condition. From 0 to 6 days after p