文档介绍:球胞白僵菌粗提物与高效***氧菊酯混合对南瓜实蝇的增效研究摘要应用球抱口僵菌代谢粗提物与高效***氧菊酯混合对南瓜实蝇进行毒力及增效倍数的测定,了解混合剂对南瓜实蝇的防治效果及增效情况。利用球他白僵菌MZ041016菌株,通过发酵培养后提取其代谢粗提物,并将其粗提物制备成两种简单的水剂和油剂,%的高效***氧菊酯以10:1的比例混合,制成生物与化学农纱两种混合型线剂,稀释成10倍、50倍和100倍3个浓度梯度后对南瓜实蝇成虫、幼虫、蛹进行毒力测定,计算出增效倍数。结果表明:随浓度梯度增大和时间的推延,累计校正死亡率均逐渐增大;;增效倍数逐渐增大。约后第6d,高浓度油剂混合剂和水剂混合剂对南瓜实蝇幼虫矫正死亡率最大,为(±)%和(±)%;其次对南瓜实蝇成虫,为(±)%和(±)%;最小是南瓜实蝇蛹,为(±)%和(±)%,高浓度油剂混合剂和水剂混合剂对南瓜实蝇致死中浓度从大到小依次是蛹〉成虫〉幼虫,分别为:/L;/L;/L,油剂混合剂对南瓜实蝇成虫增效倍数最大的,,最小是水剂混合剂对南瓜实蝇幼虫,。关键词:球他白僵菌;南瓜实蝇;混合剂;增效倍数;ABSTRACTToxicitiesofBeauveriabassianatoxicsecretionmixedBeta-:wateragentandunoilagent,:%bata-pound,then,toxicitydeterminationoftoxicextractionfromadult,:Withtheconcentrationgradientofincreasingandtimedelay,Afteradministrationof6d,cumulativemortalityrategraduallyincreased;LC50isgraduallydecreased;synergismgraduallyincreased,highconcentrationofmixedoilandmixedtypewaterretainingagentonmortalityofBactrocerataularvaemaximumcorrection,Forthe(+)%and(+)%;secondlytheBactroceratauadult,as(+)%and(+)%;theminimumisBactrocerataupupa,for(+)%and(+)%.highconcentrationofmixedoilandmixedtypewaterretainingagentonBactrocerataulethalconcentrationoftheorderfromhightolowispupa>adult>larva,respectively:/L;/L;/L,,:Beauveriabassiana;Bactroceratau;mixedreagent; 2材料与方法 3