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寄生虫实验三.ppt

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寄生虫实验三.ppt

上传人:luyinyzha 2018/1/2 文件大小:741 KB

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文档介绍:Experiment III   Examination of Fecal samples
粪便检查法
1. Place a drop of isotonic saline on a slide.
a small piece of feces with a toothpick
and stir it into the saline.
3. Add a coverslip or not, and examine
microscopically.
一、直接涂片法(Direct Smear )
二、饱和盐水浮卵法(Fecal Flotation)
Mix several grams (a teaspoonful) of feces with the flotation solution in a plastic cup.
Strain the mixture through a cuprum strainer (60 mesh) into another plastic cup.
Allow the flotation to stand for at least 5 minutes.
Take a few drops of the surface of the solution with cuprum wire loop on a microscope slide, examine them under the mecroscope with the 10× lens.
三、沉淀法(Fecal Sedimentation)
Mix about 100ml of water with about 10g of feces and place in a plastic cup.
Strain the mixture through a cuprum strainer (60 mesh) into another cup.
Allow mixture to sit for 10 minutes and then decant the supernatant.
Add more water, mix, and repeat the sedimentation procedure until the supernatant will be very clear.
Stir remaining mixture and place a few drops on a microscope slide, examine them under the mecroscope with the 10× lens.
家禽体内的寄生虫卵























牛体内的寄生虫卵
1. 大片吸虫卵