文档介绍：※生物工程食品科学 2010, Vol. 31, No. 17 323
真姬菇液体菌种培养基筛选和摇瓶发酵
条件的研究
王丽娟,刘林德,卜庆梅,王爱云,王淑芳
(鲁东大学生命科学学院,山东烟台 264025)
摘要:采用摇瓶培养法对真姬菇液体菌种培养基筛选和发酵条件进行研究。研究表明,真姬菇液体菌种最佳组合
培养基为:葡萄糖 3g/100mL、玉米淀粉 1g/100mL、黄豆粉 、(NH4)2SO4 、KH2PO4 、
MgSO4·7H2O 和 VB1 10mg/L。真姬菇摇瓶发酵最佳条件为:起始 pH 值为 ,500mL 锥形瓶装液量
140~150mL,摇床转速 180r/min,温度 26℃,二级摇瓶发酵时间 72h,接种量 10%~12%。
关键词:真姬菇;培养基筛选;发酵条件
Screening of Optimal Liquid Medium for Hypsizigus marmoreus and Optimization of Its Shake
Flask Incubation Conditions
WANG Li-juan,LIU Lin-de,BU Qing-mei,WANG Ai-yun,WANG Shu-fang
(School of Life Sciences, Ludong University, Yantai 264025, China)
Abstract: In this study, position and process conditions for the liquid-state incubation of Hypsizigus marmoreus
in a shake flask were explored. The optimal position was found to consist of 3 g/100 mL glucose, 1 g/100 mL corn
starch, g/100 mL soybean cake, g/100 mL (NH4)2SO4, g/100 mL KH2PO4, g/100 mL MgSO4·7H2O and 10 mg/L
Vitamin B1. Initial pH of , medium volume contained in a 500 mL flask of 140-150 mL, shaking speed of 180 r/min, incubation
temperature of 26 ℃, incubation time of 72 h and inoculum size of 10%-12% were found optimal.
Key words:Hypsizigus marmoreus;medium selection;incubation conditions
中图分类号:;S646 文献标识码:A 文章编号:1002-6630(2010)17-0323-04
真姬菇(Hypsizigus marmoreus)又名玉