文档介绍:Repertor:Xiaoning Yuan
Noncoding RNA
,Cheng G,,et al,2007
Noncoding RNA
Introduction
Classification
Research method
Mechanism
The relationship with major disease
Noncoding RNA(ncRNA) is a kind of transcripts without the ability of coding proteins,which exist widely in highly amount of the ncRNAtranscripts is up to 98% of the whole genome’s transcripts. The difference in molecular structure and expressive pattern from other kinds of RNA endows them specific biological functions. Although exact functions of most ncRNA are not well has been demonstrated that ncRNA was associated with some human diseases.
18—30nt: miRNA,siRNA,piRNA
30—200nt: rRNA,tRNA,snRNA, snoRNA,SLRNA,SRPRNA
More than 200nt:mRNA-like noncoding RNA and so on
method
Method for identification:
bioinformation
establish specific cDNA libraries
DNA Tiling chip
Identification
method
Method for reserching function:
Clustering methed
Nock out
4. Mechanism
a. Noncoding RNA can interact with DNA, for example, noncoding RNA can modify chromosome configuration, and then affect transcription activity of local region of DNA.