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. Flash Column Chromatography Guide
Overview:
Flash column chromatography is a quick and (usually) easy way to plex
mixtures pounds. We will be performing relatively large scale separations in , around
g pound. Columns are often smaller in scale than this and some of you will experience
these once you move into the research lab. Column chromatography uses the same principles
discussed in the TLC Handout, but it can be used on a preparative scale. We will be running flash
columns since we will pressed air to push the solvent through the column. This not only
helps provide better separation, but it also cuts down on the amount of time required to run a
column.
Reading:
For an excellent description, see LLP pages 205–214.
Preparing and Running a Flash Column:
1) Determine the dry, solvent-free weight of the mixture that you want to separate.
2) Determine the solvent