文档介绍：Characterization of production of Paclitaxel and related Taxanes in Taxus Cuspidata Densiformis suspension cultures by LC, LC/MS, and LC/MS/MS
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PLANT TISSUE CULTURE
Ⅰ. Potential of Plant cell Culture for Taxane Production
Several alternative sources of paclitaxel have been identified and are currently the subjects of considerable investigation worldwide. These include the total synthesis and biosynthesis of paclitaxel, the agriculture supply of taxoids from needles of Taxus species, hemisynthesis (the attachment of a side chain to ic precursors of paclitaxel such as atin Ⅲ or 10-atin Ⅲ), fungus production, and the production of taxoids by cell and tissue culture. This reciew will concentrate only on the latter possibility.
Plant tissue culture is one approach under investigation to provide large amounts and a stable supply of pound exhibiting antineoplastic activity. A process to produce paclitaxel or paclitaxel-pounds in cell culture has already been parented. The development of fast growing cell lines capable of producing paclitaxel would not only solve the limitations in paclitaxel supplies presently needed for clinical use, but would also help conserve the large number of trees that need to be harvested in order to isolate it. Currently, scientists and researchers have been essful in initiating fast plant growth but with limited paclitaxel production o