文档介绍：复方熊胆茵陈颗粒质量标准研究
摘要:目的建立复方熊胆茵陈颗粒的质量标准。方法采用薄层色谱法鉴别制剂中的熊胆粉、茵陈、白术、甘草。采用高效液相色谱法测定制剂中牛磺熊去氧胆酸钠的含量。色谱柱:Kromail 100-5 C18 ( mm×250 mm,5 ?m);流动相:甲醇- mol/L磷酸二氢钠溶液(60∶40),pH=;检测波长:210 nm;流速:1 mL/min;柱温:25 ℃。结果在薄层色谱中可以检出熊胆粉、茵陈、白术、甘草。 1~ mg范围内与峰面积积分值线性关系良好(r= 98),%,RSD=%(n=6)。结论该方法简便、准确、专属性强,可以有效控制复方熊胆茵陈颗粒的质量。
关键词:复方熊胆茵陈颗粒;质量标准;薄层色谱法;高效液相色谱法
DOI:.1005-
中图分类号: 文献标识码:A 文章编号:1005-5304(2013)09-0052-03
Study on Quality Standard of Fufang Xiongdan Yinchen Granule LI Huang1, XU Wei1, ZHENG Hai-yin2, HONG Zhen-feng2, XU Shu-yu1 ( of Pharmacy, Fujian University of TCM, Fuzhou 350122, China; of Integrated Medicine, Fujian University of TCM, Fuzhou 350122, China)
Abstract:Objective To establish the quality standard for Fufang Xiongdan Yinchen Granule. Methods TLC was employed to identify Pulvis ellis urs, Capillary Wormwood Herbin, Atractylodis Macrocephalae Rhizoma and Glycyrrhizae Radix, and HPLC was used to determine the content of sodium tauroursodeoxycholic acid in Fufang Xiongdan Yinchen Granule. Kromail 100-5 C18 column ( mm× 250 mm, 5 ?m) in an oven at 25 ℃ was need, with a mobile phase consisting of methanol- mol/L sodium dihydrogen phosphate solution (60∶40, pH=) and UV detector at 210 nm, the flow rate was mL/min. Results Pulvis ellis urs, Capillary Wormwoo