文档介绍：based leaf stalk have 4-5 buds per a is strong and deep green from the based bud and the based leaf stalk ,but it is weak and pale yellow from axillary bud of based the based leaf stalk is the best material in adventitious buds induction and growth.
The best basis medium for adventitious buds induction and growth is MS; BA can effectively stimulate adventitious formation on test-tube plant leaves, and the optimum concentration of BA is ·L-1, but KT has no action on it. More buds can be induced on the medium with NAA.
Stage of inducing roots and transplant.
Better stout plantlets are obtained at MS+·L-1+·L-1. In the basis media,B5 is more beneficial than MS and 1/2MS to roots induction as well as growth. NAA is more effective to root induction than IBA. The most suitable medium for roots induction is MS+. The survival rate of transplanting is the relative high after plantlets were transplanted in the soil of one thirth of humus.
Measures of anti-browning
It is knowed that effect of different anti-browning is various to growth of bud from selection of Antioxidantin of Aconitum Sinomontanum. The most suitable anti
-browning is Vc(1000mg·L-1) and cut of explant is superficial brown,bud and leaf is
deep green and growth of its is good. Effect of PA(100mg·L-1)and AC(800mg·L-1) is better for anti-browning. Effect of different anti-browning is various to growth of callus too. The best anti-browning is Vc(400mg·L-1) and the fresh weight ratio of callus is highest,growth of callus is good, callus is yellow-white. Effect of PA (100mg·L-1) and AC(800mg·L-1) is better for anti-browning ,but the fresh weight ratio of callus is low.
Key words: Aconitum Smomontanum ; plant tissue culture; callus; adventitious bud; browning; rapid propagation.
研究生:廖天江
IV 专业:植物学
指导老师:张继研究方向:资源植物学
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