文档介绍：中国药科大学
本科毕业论文
论文题目人甲状旁腺素活性片段前体物制备工艺的研究
英文题目 Research on Preparation of Human Parathyroid
Hormone Fragment Analogue
专业生物技术
院部生命科学与技术学院
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指导教师
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完成场所中国药科大学酶工程实验室
论文工作时间: 年月至年月
人甲状旁腺素活性片段前体物制备工艺的研究
目录
摘要………………………………………………………………………1
前言………………………………………………………………………2
实验原理………………………………………………………3
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第二章试剂与仪器……………………………………………………4
试剂……………………………………………………4
仪器……………………………………………………4
第三章实验方法………………………………………………………5
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第四章实验结果………………………………………………………6
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第五章讨论……………………………………………………………
参考文献…………………………………………………………………7
致谢………………………………………………………………………9
人甲状旁腺素活性片段前体物制备工艺的研究
摘要:目的提高人甲状旁腺素活性片段前体物Pro-Pro-[Arg11]hPTH(1-34)-Pro-Pro的分离纯化效率。方法①通过均匀设计法寻求目的融合蛋白的酸水解条件(温度、时间、蛋白含量,盐酸浓度);进行二次酸水解。②使用核酸蛋白检测仪及紫外可见分光光度计检测210nm,280nm处吸收峰,收集样品峰,对比含量及纯度差异,确定最佳检测波长。结果①酸水解最佳条件没有得到改进;②检测波长设在210nm比设在280nm更灵敏、更可靠。结论①二次酸水解可提高水解效率;②检测波长设在210nm处可提高分离纯化效率。
关键词:人甲状旁腺素,活性片段前体物;骨质疏松;纯化;酸水解;柱层析
Research on preparation of human parathyroid
Hormone fragment analogue
Abstract:Objective To improve the separation and purification rate of human parathyroid hormone fragment analogue Pro-Pro-[Arg11]hPTH(1-34)-Pro-Pro. Methods ①To seek the conditions for acid hydrolysis of target fusion protein through uniform design;to carry out acid hydrolysis for a second time. ②To detect the assimilation peak and the sample collecting peak at 210nm and 280nm with the nucleic acid protein surveymeter and the ultraviolet visible light photometer, and to contrast the differences in contents and purity to make sure the best wavelength. Results ①No progress was made in seeking the best conditions of acid hydrolysis; ②Set up the detecting wavelength at 210nm was more sensitive and credible then at 280nm. Conclusions ①Carrying out acid hydrolysis for a second time can impro