文档介绍：盐胡芦巴HPLC指纹图谱研究
许小华,杨云*,刘富岗,肖功胜
(河南中医学院药学院,河南郑州 450008)
摘要:目的建立盐胡芦巴的HPLC指纹图谱分析方法。方法采用高效液相色谱技术,phenomenex Hydro-RP 80A(×250mm,5μm)色谱柱,乙腈-水为流动相进行等度与梯度洗脱,检测波长211nm,·min-1,柱温为40℃。结果建立了以17个共有峰为特征指纹信息的HPLC指纹图谱,发现各产地采用规范化炮制工艺制备的盐胡芦巴指纹图谱无明显差异。结论该指纹图谱方法准确可靠,重现性好,可作为盐胡芦巴内在质量评价的依据。
关键词:盐胡芦巴;HPLC;指纹图谱
Study on Fingerprints of saline semen Trigonellae by HPLC
XU Xiao-hua ,YANG Yun* , LIU Fu-gang,XIAO Gong-sheng
(College of Pharmacy, Henan University of traditional Chinese medicine, Zhenzhou )
ABSTRACT:OBJECTIVE To establish the HPLC fingerprints of saline semen Trigonellae. METHODS HPLC analysis was performed on a phenomenex Hydro-RP 80A(×250mm,5μm) with mobile phase acetonitrile-water under isocratic and gradient elutions,and detection wavelength was 211nm,the flow rate was 1ml·min-1 ,column temperature was 40℃.RESULTS HPLC fingerprint of 17 main peaks was was found that the HPLC of preparation of standardized processing saline semen Trigonellae from different places have no significant The method is reliable,accurate and can be used for quality control of saline semen Trigonellae.
KEY WORDS: saline semen Trigonellae; HPLC; fingerprint
胡芦巴为豆科植物胡芦巴Trigonellae foenum-gracum ,性味苦,温,归肾经,具有温肾,祛寒,止痛等功效,主治肾脏虚冷,小腹冷痛,小肠疝气,寒湿脚气,是我国传统的温肾补阳药物。现代化学和药理实验证明[1],胡芦巴有多方面的生物活性,
基金项目:河南省重大科技攻关项目(0522030200)
作者简介:许小华,男,硕士研究