6,8-二-三氟甲基-7-乙酰基白杨素抗肝癌作用实验研究.doc

文档介绍：6,8-二-三氟甲基-7-乙酰基白杨素抗肝癌作用实验研究
夏红张燕琴秦勇刘华清周建国董琳曹建国1,2
(1南华大学肿瘤研究所, 衡阳 421001
2南华大学药物药理研究所,衡阳 421001)
[摘要] 目的:研究6,8-二-三氟甲基-7-乙酰基白杨素(dFMAChR)体内外抗肝癌作用。方法: MTT比色法测定dFMAChR对体外培养人肝癌细胞Hep G2细胞和人胚肝细胞L-02细胞增殖的影响;平皿克隆形成法及软琼脂克隆形成法测定dFMAChR对体外培养Hep G2细胞的锚定依赖性及非锚定依赖性生长作用;PI染色流式细胞术(FCM)分析dFMAChR对Hep G2细胞周期的影响;人肝癌裸鼠异种移植瘤模型治疗实验评价dFMAChR治疗人肝癌的有效性。结果:dFMAChR抑制体外培养人肝癌Hep G2细胞增殖和生长,其效价强度高于先导化合物白杨素(ChR),而对人胚肝(L-02)细胞的毒性小,。,, G2细胞48h后,%、%、%,较溶媒对照组和ChR %%有显著提高,呈现G1期阻滞现象。人肝癌裸鼠异种移植瘤模型治疗实验结果显示:dFMAChR对人肝癌异种移植瘤生长具有显著抑制作用,20、40、80mg/%,%%。结论:dFMAChR具有人肝癌治疗作用。
关键词肝肿瘤;白杨素; 6,8-二-三氟甲基-7-乙酰基白杨素;治疗作用
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作者简介:夏红,29岁,硕士研究生。研究方向:肿瘤药理学。
Email:xiahong998@
曹建国(1956-),男,教授,硕士生导师。研究方向:肿瘤药理学。通讯作者。Tel:[phone] Email:caojianguo2005@
基金项目:湖南省卫生厅医药卫生科研项目编号 B2006-102
Experimental study on therapeutic effect of human lever carcinoma by 6, 8 -ditrifluoromethy-7-acetoyChrysin
XIA Hong, ZHANG Yan-Qin, QIN Yong, LIU Hua-Qin, ZHOU Jian-Guo, DONG Lin, CAO Jian-Guo1,2
1Institute of Cancer research, Nanhua University, Hengyang, 421001, China
2Institute of Pharmacy & Pharmacology, Nanhua University, Hengyang 421001, China
[Abstract] Objective: To study on the therapeutic effect of human lever carcinoma by 6,8-ditrifluoromethy-7-acetoy chrysin (dFMAChR) in vitro and vivo. Methods: Human lever carcinoma (Hep G2) cells were cultured in vitro. The proliferative inhibition of dFMAChR on Hep G2 cells were examined by MTT assay. Clone-formation assay in plate and soft agar was used to test inhibition of clone formation of Hep G2 by dFMAChR. Flow cytometry(FCM) using PI staining was used to analyse the apoptosis and cell cycle change after treatment with dFMAChR . Human lever carcinoma xenograft in nude mouse model was established to investigate the anti-tumor effect of dFMAChR in vivo. Results: dFMAChR inhibited proliferation and growth of Hep G2 cells in