文档介绍：,
江西农业大学学报 2012 34 5 1049 - 1052 http / /xuebao. jxau. edu. cn
Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com
酶法辅助提取迷迭香中三萜酸的工艺研究
李开泉1,2 ,陶华蕾2
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1. 江西省天然药物活性成分研究重点实验室江西宜春 336000 2. 宜春学院化学与生物工程学院江西宜春
336000
摘要为探讨迷迭香中三萜酸的最佳提取工艺条件,采用纤维素酶法,通过单因素试验和正交设计对迷迭香中
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三萜酸的提取工艺进行研究。酶解法提取迷迭香中三萜酸的最佳条件为加酶量 6. 5 mg/g 酶解时间为 2. 5 h 酶解
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温度 45 ℃体系 pH 值 6. 5。在此条件下所得迷迭香中三萜酸干质量为 4. 58 g。纤维素酶能有效地破坏细胞
, , , ,
壁加速有效物质的释放纤维素酶酶解条件温和工艺简单值得推广应用。
关键词迷迭香三萜酸纤维素酶提取工艺
中图分类号R284. 1 Q946. 33 文献标志码A 文章编号1000 - 2286 2012 05 - 1049 - 04
Study on Extraction Process of Triterpene Acids form Rosemary
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1 2 , 2
LI Kai-quan TAO Hua-lei
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1. Key Laboratory for Research on Active Ingredients in Natural Medicines of Jiangxi Province Yichun
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336000 China 2. Chemical and Biological Engineering Department of Yichun University Yichun 336000
China
Abstract To explore the optimum conditions for extraction of Triterpenoid acid in rosemary. Use of cellu-
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lose enzyme to research the extraction process of triterpenoid acid from rosemary by single factor experiments
and orthogonal design. The best conditions for enzymatic extraction of triterpenoid acids from rosemary the a-
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mount of enzyme 6. 5 mg /g hydrolysis time 2. 5 h reaction temperature 45 ℃ pH value 6. 5. Under these
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conditions the proceeds of trit