文档介绍：结核分支杆菌特异性抗原重组蛋白MPT63检测系统的建立
程李克生张润玲
【摘要】目的建立规范的结核分枝杆菌(mycobacterium tuberculosis,MTB)特异性抗原重组蛋白MPT63酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)检测系统。方法采用方阵法确定蛋白包被浓度和酶标二抗浓度,以ROC(Receiver Operating Characteristic)曲线法确定MPT63蛋白 ELISA法检测的cut-off值。探讨规范的ELISA检测系统建立的方法。结果 ELISA检测系统MPT63蛋白包被浓度1﹕128000,酶浓度1﹕1000时为最佳;在cut--,分别是75%和 %。结论用方阵法确定蛋白包被浓度和酶标二抗浓度,并以ROC曲线法确立cut-off值是建立规范化的ELISA检测系统的关键环节。
关键词重组蛋白MPT63;ELISA; ROC曲线; cut-off值
Establishment detection system of bination protein MPT63 on mycobacterium tuberculosis specific antigen CHENG Guo-ping,Department of Clinical Laboratory, The First Affiliated Hospital of Henan University of Science and Technology,Henan 471000,China LI Ke-sheng Medical Science Research Institute of Gansu province Biotechnology Center,Zhang Run-ling The original Department of Clinical Laboratory ,The Second Affiliated Hospital of Lanzhou University Laboratory
【Abstract】Objective To set up a standard testing system of mycobacterium tuberculosis specific antigen- binated MPT63 protein by enzyme-linked immunosorbent assay method. Methods The square matrix method is used to determine the concentration of coated protein and the concentration of enzyme labeling antibody. Receiver Operating Characteristic method is used to determine the cut-off value of ELISA detected MPT63 approach a establishment of standa