文档介绍：兔病毒性出血症鸡源高免蛋黄抗体的研究与应用
﹡王孝友1 徐刚3 何洪章2 孔路军1 曾秀1 黄伟3
(
402460)
摘要:用兔病毒性出血症抗原免疫产蛋鸡,收集高免蛋,精制蛋黄抗体(IgY),采用血凝抑制试验(HI)测定IgY的最高滴度为15log2。通过IgY的被动免疫保护试验证实,IgY滴度稀释至13log2时肌肉注射体重2Kg-,(血凝价HA为9log2)的攻击。临床应用效果显示:IgY(HI为13l0g2)对发病兔进行紧急防治,%—%,能迅速控制疫情。
关键词:兔病毒性出血症;蛋黄抗体
Research and Appalication of Rabbit Haemorrhagic Disease virus
High-lever Immunized yolk Antibody
﹡WANG Xiao-you1,XU Gang3,HE Hong-zhang2,KONG Lu-jun1,
ZENG Xiu1,HUANG Wei3
(1 Chongqing Swine Science Academy,2 Chongqing health-forever
biotechnology limited-pany, 3 South-west
Agriculture University,Chongqing Rongchang 402460,China)
Abstract:A flow full-grown hens were immunized with inactivated rabbit haemorrhagic disease virus(RHDV).After a boosting process eggs were collected and used to extract the special yolk antibody(IgY).The maximum titer of this preparation reached 15log2 by hemagglutination inhibition assay(HI).It was confirmed through passive immunization of IgY that healthy nonimmunized rabbits whose weight varied during 2Kg and were intramuscularinjected with IgY (HI=13log2),they could resist the attact of (HA=9log2) RHDV- clinical application effect of IgY( HI= 13log2) showed the cure rate was to percent to diseased rabbits and they could control the epidemic situation im