文档介绍：抗氧化剂对猪精液冷冻保存效果的影响
杨升1 ,贾东平1, 刘家慧2
(,天津 300384; 266555)
摘要:经过试验筛选最适合的抗氧化剂剂量,添加200µM AA-2G获得冻后最高活力( )、质膜完整率(%)和顶体完整率(% ),%,与对照组精子冻后活率( )、质膜完整率(% )和顶体完整率(%)、%比较差异显著。添加深海鱼油4%可显著提高猪精子的冻后活率()、质膜完整率(40%)和顶体完整率(51% ),,与对照组精子冻后活率( )、质膜完整率(% )和顶体完整率(%)、%比较差异显著。在猪精子冷冻过程中添加200µM的AA-2G、4%的深海鱼油可显著提高猪精子的冻后活率、质膜完整率和顶体完整率,降低精子的获能数。
关键词: 猪精液;冷冻保存;抗氧化剂
Abstract::After a test screening the most suitable dose of antioxidants, AA-2G in the optimal concentration of 200μM, the optimum additive amount of deep sea fish oil is 4%. Add 200μM AA-2G freezing the highest activity (), plasma membrane integrity rate (%) and acrosome integrity rate (%), the sperm has not been able to % in the control group survival rate after frozen sperm ( ), plasma membrane integrity rate (%) and acrosome integrity rate (%), % of the difference can be significant. 4% added fish oil can significantly increase the sperm's survival rate after freezing (), plasma membrane integrity rate (40%) and acrosome integrity rate (51%), capacitated sperm, frozen sperm and the control group After the survival rate (), plasma membrane integrity rate (%) and acrosome integrity rate (%), % of the difference can be significant. Pig sperm frozen in the process of adding 200μM of AA-2G, 4