文档介绍：正交设计优选芪归通络口服液提取工艺
张建军1,李智勇1,2,庄玉坚1,2,马玲 2
(,广东广州 510095;,广东广州 510405)
摘要:目的筛选并优化芪归通络口服液提取工艺。方法以80%醇浸出物得率和芍药苷提取率为考察指标,采用正交设计多指标综合评分法优化提取工艺。结果通过正交试验优化确定的提取条件为:药材饮片以9倍水提取3次,每次40min。结论优化得到的提取工艺合理、稳定、可行。
关键词:芪归通络口服液;提取工艺;正交设计;芍药苷
The Study on Selecting the Optimal Extraction Technique of Qiguitongluo Oral Liquid by Orthogonal Design
ZHANG Jian-jun1, LI Zhi-yong1,2, ZHUANG Yu-jian1,2 , MA Ling2
(1. Guangdong Institute of Traditional Chinese Medicine, Guangzhou 510095, China;
University of Chinese Medicine, Guangzhou 510405,China)
Abstract: AM: To screen and optimize the extraction of Qiguitongluo oral liquid. Methods: Taking extraction yield of paeoniflorin and 80% alcohol extractive as investigation index, the best extraction process was evaluated by orthogonal experimental design. Results: The optimized extraction technique was adding 9 times water, extracting 40 minutes for 3 times. Conclusion: The optimum extraction was simple, reasonable and useful for further development.
Key words: Qiguitongluo oral liquid; extraction process; orthogonal design; paeoniflorin
芪归通络口服液为广东省第二中医院院内制剂,验方由黄芪、当归、赤芍等药味组成,具有益气养血,活血化瘀通络的功效,主治中风病气虚血瘀证,临床用于中风急性期或恢复期患者,对缺血性中风和出血性中风均具有良好的疗效。本试验以提取液中芍药苷的提取量和干膏得率为考察指标,采用正交试验设计对其提取工艺条件进行优选,为生产工艺参数的确定提供实验依据。
1 仪器与试药
Agilent 1100高效液相色谱仪:DAD检测器、四元梯度泵、G2170A数据处
理系统(Agilent,美(Sartorius,德国),药材(广东省第二中医院中药房),芍