文档介绍：毕业论文
题目:西伯利亚蝗基因组DNA不同
提取方法的研究
姓名:
专业: 生物技术
班级:
院(系): 生命与环境科学学院生物系
指导老师:
新疆师范大学
目录
中文摘要…………………………………………………………………………………… 3
英文摘要…………………………………………………………………………………… 4
引言………………………………………………………………………………………… 5
1 材料与方法……………………………………………………………………………… 6
实验材料…………………………………………………………………………………… 6
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SDS-蛋白酶K消化法……………………………………………………………… 7
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CTAB法……………………………………………………………………………… 9
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2结果与分析……………………………………………………………………………… 10
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3 讨论……………………………………………………………………………………… 13
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参考文献…………………………………………………………………………………… 15
西伯利亚蝗基因组DNA不同提取方法的研究
(新疆师范大学生命与环境科学学院乌鲁木齐 830054)
摘要:本实验以新疆草原蝗虫的主要危害种类——西伯利亚蝗为研究对象,分别对干制和乙醇浸泡的标本用SDS法、饱和NaCl 法和CTAB法进行了基因组DNA 的提取比较。实验结果表明,SDS法提取的总DNA 带型整齐,饱和NaCl法和CTAB法提取的标本总DNA获得率较低,在琼脂糖凝胶电泳检测中大部分有明显降解。另外,乙醇浸制的标本总DNA的获得率及质量均高于干标本。研究结果可为今后进一步开展蝗科亲缘关系分析、基因组比较和遗传多态性等分子生物学研究提供基础资料。
关键词:基因组DNA , 不同方法, 西伯利亚蝗
Studies on different extraction methods of Gomphocerus sibiricus genomic DNA
(College of Life and environment sciences ,Xinjiang Normal university, urumqi 830054)
Abstract: The experiments in Xinjiang grassland locusts main types of hazards Gomphocerus sibiricus for research, genome DNA of grasshoppers which were kept as dry sample or preserved in 100% ethanol was extracted using SDS method,Saturated NaCl law and the law CTAB extraction of DNA results showed ,SDS method brought from the total DNA, saturated Nacl law and the law CTAB extraction of total DNA in specimens of the lower rate in most Agarose gel electrophoresis testing marked degradation. In addition, ethanol college system overall DNA specimens obtained were higher than the rate and quality of the