实验中医学论文.pdf

文档介绍：《实验中医学》论文��指导老师:潘志强、刘小美、宋菊敏学生:宋欣阳(2002207202)
环磷酰***所致的小鼠证候模型的研究
——党参与参苓白术散对其血清蛋白含量的影响
摘要目的研究环磷酰***(CTX)所致的小鼠证侯模型的特点与分型。方法模
型组、治疗组 1,治疗组 2 小鼠腹腔注射 CTX(10mg/ml),,连续 2d,
治疗组 1,治疗组 2 分别以党参、参苓白术散,浓缩液进行灌胃,同时设立正常
组,同样方法注射生理盐水。测定血清中白蛋白、球蛋白、总蛋白含量,对小鼠
进行四诊辨证,观察各脏器病理图片及血液、骨髓图片,进行肝组织基因 RT-PCR
检测。结果模型组血清总蛋白含量较正常组呈下降趋势,治疗组 1、2 小鼠血清
总蛋白含量较模型组呈升高趋势,模型组血清白蛋白较正常组呈升高趋势,治疗
组 1 较模型组呈降低趋势,治疗组 2 较模型组呈升高趋势,血清球蛋白含量治疗
组、正常组均较模型组有升高趋势,但三组数据均无统计学意义(P>)。病
理图片、血液、骨髓图片、RT-PCR 结果均无明显差异。结论党参对 CTX 所致
的证侯模型可能影响不大或有正面影响,参苓白术散的治疗作用可能强于党参,
具有一定的正面影响。结合其它实验结果,推断 CTX 所致的小鼠证候模型并非
单纯的脾气虚模型,具体分型需进行进一步实验研究。
关键词:气虚证,环磷酰***,党参,参苓白术散,生化研究
A study on symptom model induced by Cyclophosphamide in mice
——Effects of Codonopsis pilosula (Dangshen) and
Shenlinbaizhu Powder on the content of serum proteinum
Abstract
Objective TO investing thecharacteristic and classification of the syndrome model
induced by cyclophosphamide (CTX) in mice. Methods The model group、
treatment group 1 and treatment group 2 mice were injected ip with per mouse
per day CTX(10mg·ml-1) and last 2 days. The treatment group 1 and treatment group
2 mice gave concentrated solution of Codonopsis pilosula (Dangshen) and
Shenlinbaizhu Powder respectively by intragastric establish the
nomal group which inject normal saline at the same the contents of serum
protein、serum globulin、serum albumin,and to distinguish their diffrences use four
physical examinations,and observe pathological samples of an、blood
samples、marrow samples,and examine the gene of liver tissue by RT-PCR. Results
Compared with the normal group mice:the Serum Protein content in the model group
mice has lower tendency but the serum albumin content has higher tendency;
Compared with the model group mice:the Serum Protein content in the two treatment
groups have higer tendency,and the serum albumin content in treatment group 1 is
lower, in treatment group 2 is higher. the Serum g