文档介绍：2012-12-25
DOI: .
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应用与环境生物学报 Chin J Appl Environ Biol 2012,18 (6): 943~947
4种重金属离子对克氏原螯虾(Procambarus clarkii)
NAGase活力的影响*
张伟妮陈欣颖黄小红** 胡崇伟黄一帆**
(福建农林大学中西兽医结合与动物保健福建省高校重点实验室福州 350002)
摘要
研究了Ag+、Pb2+、Zn 2+、Hg2+等重金属离子对克氏原螯虾(Procambarus clarkia)N-乙酰-β-D-氨基葡萄糖苷酶
(NAGase)活力的影响,结果表明:4种重金属离子对酶活力均有不同程度的可逆抑制作用,其中Hg 2+、Zn 2+对酶的抑
制作用较强,表现为反竞争性抑制,对结合酶(ES)的抑制常数 KIS分别为 mmol/L和 mmol/L;而Ag+、Pb2+对
酶有先激活后抑制的作用,其中Ag+对酶的抑制作用类型表现为反竞争性抑制,其抑制常数 mmol/L,Pb2+对酶
的抑制作用类型表现为非竞争性抑制,其抑制常数 KI为 mmol/L. 图9 参11
关键词克氏原螯虾;N-乙酰-β-D-氨基葡萄糖苷酶;酶活力;金属离子;作用机理
CLC
Effects of Four Heavy Metal Ions on NAGase of Procambarus clarkii*
ZHANG Weini, CHEN Xinying, HUANG Xiaohong**, HU Chongwei & HUANG Yifan**
(University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fujian
Agriculture and Forestry University, Fuzhou 350002, China )
Abstract The effects of four heavy metal ions (Ag+, Pb2+, Zn 2+, Hg2+) on N-acetyl-β-D-glucosaminidase from Procambarus
clarkii were studied. The results showed that four heavy metal ions reversibly inhibited the activity of enzyme in varied degree.
Hg 2+ and Zn 2+ had stronger inhibiting effects, and they were both petitive inhibitors of this enzyme. The inhibition
constants of enzyme-plex (KIS) were mmol/L and mmol/L, respe