文档介绍：毕业设计说明书
年产3万吨红曲色素生产工艺设计
摘要:红曲色素是一种优良的色素,是红曲在菌体生长代谢过程中产生的天然色素,具有较高的营养价值和药用价值,目前广泛应用于医药、食品、饲料和化妆品等领域。
本设计以从土壤中筛选的红曲霉为出发菌株,通过紫外线超声波复合诱变选育出红曲色素高产菌株,通过摇瓶筛选和稳定性试验,得到稳定性高产红曲色素的突变菌株。在此基础上,尝试了三级发酵的发酵方式,优化了培养基,确定了发酵工艺条件:接种菌龄20h、接种量5%、、培养最适温度32℃及发酵时间96h,并选择了合适下游提取工艺。设计的生产工艺稳定、简便,发酵后所得的红曲色素产量达到300g/L。
关键词:红曲霉,红曲色素,发酵,诱变
Abstract:Monascus pigment is a good natural pigment, which Monascus pigment in the cell growth in the metabolism process produces, has high nutritional value and medicinal value, is now widely used in the fields of medicine, food, feed and cosmetics etc
The design of the screening from the soil of Monascus as starting strain, through ultraviolet pound mutation breeding of the monascus pigment producing strains, through shake flask screening and stability test, the stability of mutant strains obtained high yield of Monascus pigment. On this basis, try the fermentation level three fermentation, optimization of the culture medium, fermentation conditions were determined: inoculating age 20h, 5% inoculation quantity, initial , culture optimum temperature is 32 ℃ and the fermentation time was 96h, and select the appropriate downstream extraction process. Design of production process stability, simple, after fermentation of Monascus pigment yield e reached 300g/L.
Keywords: Monascus, Monascus pigment, fermentation, mutation
目录
1引言 1
概述 1
发酵方式 1
红曲色素合成机理 1
国内外研究现状 2
国内研究现状 2
国外研究现状 3
本课题的意义 4
2红曲霉菌种的选育 5
5
5
5
诱变选育 5
5
6
稳定性试验 6
菌种保藏 6
3 培养基 7
7
培养基的设计 7
碳源 7
氮源 7
无机盐及微量元素 8
4 灭菌 9
仪器灭菌 9
培养基灭菌 9
10
10
空气除菌 10
发酵罐灭菌 10
5 种子扩大培养和装料发酵 11
斜面培养 11
一级种子培养 11
二级种子培养 11
装料发酵 12
6 发酵控制 13
pH值的控制 13
溶氧的控制 13
补料工艺的控制 13
14
葡萄糖与蛋白胨 14
7 下游加工 16
16
16
16
胞外色素 16