文档介绍：通心络胶囊干预载脂蛋白E基因敲除小鼠MMP-1、MMP-9及TIMP1的研究
Study of Tongxinluo Capsule in Intervention of Apolipoprotein E Gene Knocking Out MMP-1, MMP-9 and TIMP1 in Mice
韩旭李七一夏卫军赖仁胜陈小虎郭宏敏赵惠
Han Xu, Li Qiyi, Xia Weijun, Lai Rensheng, Chen Xiaohu, Guo Hongmin, Zhao Hui
江苏省中医院(中国 210029)
Traditional Chinese Medicine Hospital in Jiangsu Province ( China, 210029)
中图分类号: 文献标识码:A 文章编号:1818-0086(2010)04
摘要:目的通过通心络胶囊对载脂蛋白E基因敲除〔ApoE(-/-)〕模型组小鼠基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶组织抑制剂1(TIMP1)水平等指标的干预变化,探究其治疗冠心病(CHD)的机理。方法将40只ApoE (-/-)小鼠作为实验组,建立冠状动脉粥样硬化(AS)模型,随机分为模型组等5组,分别给予辛伐他汀及不同剂量的通心络胶囊进行干预,另选8只同系的小鼠作为正常对照组。通过对模型组小鼠血清MMP-1、MMP-9 和TIMP1水平的观察,比较分析通心络胶囊对ApoE (-/-)小鼠冠状AS的调节作用并探讨其机理。结果通心络胶囊高剂量组小鼠血清MMP-1和MMP-9水平均低于模型组,而TIMP1水平高于模型组,与模型组相比,均有统计学意义(P<<)。通心络胶囊中剂量组小鼠血清MMP-9水平低于模型组,与模型组相比有统计学意义(P<)。结论通心络胶囊具有抑制血管重构和稳定斑块的作用。
关键词:通心络胶囊;ApoE(-/-)小鼠;冠状AS;MMP-1;MMP-9;TIMP1;干预
Abstract Objectives To investigate mechanism of Tongxinluo Capsule managing coronary heart disease (CHD) by observing changes resulting from intervention of apolipoprotein E gene knocking out [ApoE (-/-) ] MMP-1, MMP-9 and TIMP1 in mice model. Method 40 ApoE (-/-) mice, as experimental group, were used to establish coronary arteriosclerosis (AS) model and then were randomly divided into 5 model groups. Simvastatin and Tongxinluo Capsule at different dosage were administrated to mice respectively as intervention an