泰乐菌素降解菌的分离鉴定及其系统发育分析.pdf

文档介绍：11 1
2012-12-25 DOI: . /
应用与环境生物学报 Chin J Appl Environ Biol 2012,18 ( 6 ) : 1038~1042
泰乐菌素降解菌的分离鉴定及其系统发育分析*
刘力嘉 1 马玉龙 2** 谢丽2 张新莎2
(1宁夏大学农学院银川 750021)
(2宁夏大学天然药物工程技术研究中心银川 750021)
摘要发酵法生产泰乐菌素过程中产生的大量废弃药渣,因残留泰乐菌素的存在,会对环境带来不利影响. 采用微
生物法降解药渣中残留的泰乐菌素. 结果表明:从堆放泰乐菌素药渣附近土壤中分离筛选到1株高效降解泰乐菌素的
菌株,经 16S rDNA鉴定为无丙二酸柠檬酸杆菌(Citrobacter amalonaticus). 该菌为好氧型革兰氏阴性杆菌,菌落直径
~ mm,可利用多种糖类、醇类和氨基酸作为碳源或氮源,具有较强的耐盐、耐酸、耐碱能力,对温度的适应范围
较宽. 用该菌株在30 ℃下处理含50 mg/L泰乐菌素培养基72 h,培养基中未检到泰乐菌素的存在. 说明利用微生物法可
有效降解药渣中残留的泰乐菌素. 图4 表3 参20
关键词泰乐菌素;无丙二酸柠檬酸杆菌;16S rDNA;系统发育分析
CLC X172 : Q344+.3
Isolation, Identification and ic Analysis of a Tylosin-degrading Strain*
LIU Lijia1, MA Yulong2**, XIE Li2 & ZHANG Xinsha2
(1College of Agriculture, Ningxia University, Yinchuan 750021, China)
(2Engineering Research Center for Natural Medicine, Ningxia University, Yinchuan 750021, China)
Abstract A mass of pharmaceutical waste is produced in the process of tylosin production using microbial fermentation, and
has a deleterious effect on the environment because of the existence of tylosin residue. The aim of this study was to investigate
the degradation of tylosin residue in pharmaceutical waste by microbiological method. A tylosin-degrading strain was isolated
from the soil deposited by tylosin pharmaceutical waste and identified as Citrobacter amalonaticus base