文档介绍：LC-VWD and LC-MS analysis of the major content of prepared root of P. multiflorum
Feng Zhang a,b, Wansheng Chen b, Yang Yang a,b, Fei Cai a,b, Shouhong Gao b, Lianna Sun a,*
a School of Pharmacy, Second Military Medical University, Guohe Road, Shanghai 200433, PR China
b Department of Pharmacy, the affiliated Changzheng Hospital of Second Military Medical University, Fengyang Road, Shanghai 200003, PR China
*To whom correspondence should be addressed.
Lianna Sun, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China.
Tel: +86-021-25074574; fax: +86-021-25074574.
E-mail address: sssnmr@
Abstract
In this study, a high performance liquid chromatography (HPLC) coupled with a variable wavelength detector (VWD) has been developed for the simultaneous determination of three anthraquinones (including emodin, physcion, emodine-8-O-β-D-glucoside) and one stilbene ((E)-2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside) in prepared roots of Polygonum multiflorum Thunb. (P. multiflorum). It is widely used in the prescription of traditional Chinese medicine (TCM), and also edible as an invigorant. This method was simple, reliable and reproducible, and was essfully applied to determine the four pounds in the prepared P. multiflorum for the quality control. In addition, it was the first time reported to identify another one naphthalene derivation and three stilbenes by this ESI/MS method.
Keywords: Polygonum multiflorum Thunb.; HPLC-VWD; emodin; physcion; emodine-8-O-β-D-glucoside; (E)-2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside
1. Introduction
Polygonum multiflorum Thunb. (P. multiflorum), monly used traditional Chinese medicine (TCM) with low toxicity for thousands of years, contains anthraquinones and stilbenes as its major constituents. mon anthraquinones, free and glycosidated, such as emodin (EM), physcion (PH), emodine-8-O-β-D-glucoside (EMG) (Fig. 1), are mostly applied in traditional therapy [1]. p