文档介绍：松针不同提取部位体外抗肿瘤作用的实验研究
周微a,郑晓珂b,王小兰a,冯卫生a
(河南中医学院,;,郑州 450008)
摘要:目的探讨松针不同提取部位的体外抗肿瘤作用。方法采用系统溶剂萃取法将松针醇提物进行部位分离,得到石油醚、乙酸乙酯、正丁醇及水提取部位,通过四甲基偶氮唑盐比色法(MTT法)观察各提取部位对人肝癌细胞株SMMC-7721、HepG2,人乳腺癌细胞株MCF-7,人前列腺癌细胞株PC-3细胞增殖的抑制作用。采用流式细胞术分析细胞周期分布,初探松针抗肿瘤作用机理。结果石油醚部位对肿瘤细胞的抑制作用较强,在一定剂量范围内呈现出时间和剂量依赖性;乙酸乙酯部位的作用次之;正丁醇和水部位的抑制作用不明显。SMMC- mg·ml-1、 mg·ml-%、%,它对该细胞增殖的抑制是将细胞周期阻滞在G2-M期。结论松针提取物在体外具有抗肿瘤作用,石油醚部位呈现出较强的抑制作用,其体外抑制SMMC-7721细胞增殖的机制可能与松针提取物的细胞毒作用、诱导细胞凋亡和抑制细胞分裂有关。
关键词:松针;肿瘤细胞株;MTT法;流式细胞术
Experimental Study on Antitumor Effect of Different Fractions of Pine Needles in vitro
ZHOU Weia,ZHENG Xiao-keb,WANG Xiao-lana,FENG Wei-shenga
(Henan University of Traditional Chinese Medicine, of Pharmacy; Medical College,Zhengzhou 450008)
Abstract: Objective To study the antitumor effect of different fractions of pine needles in vitro. Methods Pine needles were extracted and separated by the technique of solvent- assay was used to observe the inhibition of extracts on the proliferation of human hepatocellular carcinoma SMMC-7721, HepG2, breast carcinoma MCF-7 and prostatic carcinoma PC- rates and cell cycle distribution were detected by flow cytometry Petroleum ether extract from pine needles possesses the role of antitumor effect in cell culture. The inhibitory effect on the proliferation of tumo