文档介绍：Study on Preliminary Mechanism Apoptosis in HepG-2 by CSA
Yu Lei1,2,3 Mo Ke2,3 Wang Wei2,3 Cui Rong-tian2,3 JI Yu-bin1,2,3 Zou Xiang1,2,3
( of Research and Development on Life Sciences and Environmental Sciences, Harbin University merce, Harbin 150076, China; of Materia Medica and Postdoctoral Programme of Harbin University merce, Harbin 150076, China; Reseach Center of Natural Anticancer Drugs,Ministry of Education)
Abstract: Objective To study on the mechanism of killing and apoptosis inducing effect of total alkaloid in the CSA(Capparis spinosa L. alkaloid, CSA)on human heptocarcinoma cell Line HepG-2. Methods The killing effect of the CSA on human heptocarcinoma cell Line HepG-2 was measured by MTT method. Morphological observation of the HepG-2 cells pleted by fluorescence microscope. The apoptosis inducing effect and changing of mitochondria membrane potential of the CSA on the HepG-2 cells were measured by flowcytometry. In addition, effect of intracellular Ca2+ level of the CSA on the HepG-2 cells was studied by laser confocal microscope. Results The CSA has obvious cytotoxicity on the HepG-2 and seems to be dose-dependent, and its IC50 value is . The HepG-2 cells have characteristic morphologic changes of apoptosis by the function of CSA, and the apoptosis percentage is higher than the natural one. The progress of cells cyc