丙酮丁醇梭菌Clostridium acetobutylicum CICC 8012 发酵鲜芭蕉芋生产丁醇.pdf

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2013-02-25 DOI: . /
应用与环境生物学报 Chin J Appl Environ Biol 2013,19 ( 1 ) : 54-60
丙酮丁醇梭菌Clostridium acetobutylicum 8012
发酵鲜芭蕉芋生产丁醇*
龙飞1, 2, 3 靳艳玲1, 3 赵云4 郜晓峰1, 2, 3 李宇浩 1, 2, 3 陈谦1, 2, 3 赵海1,3**
(1中国科学院成都生物研究所成都 610041)
(2中国科学院大学北京 100049)
(3中国科学院环境与应用微生物重点实验室成都 610041)
(4四川大学生命科学学院成都 610064)
摘要以非粮作物鲜芭蕉芋为原料, 8012发酵生产丁醇. D),选
取初始糖浓度、接种量、中性红、乙酸铵为主要影响因素,对发酵条件进行优化,建立以丁醇产量为响应值的数学模
型. 对模型求解得到: g/L,接种量为 %,中性红为 g/L,乙酸铵为 g/L时,最终的丁醇产
g/L. 验证实验结果表明,在优化条件下丁醇发酵产量达到 g/L,证明了模型可靠有效. 图3 表5 参24
关键词丁醇;鲜芭蕉芋;丙酮丁醇梭菌;响应面法;发酵
CLC TQ923 : S216
Optimization of Butanol Production from Canna edulis Ker by Clostridium
acetobutylicum Using Response Surface Methodology*
LONG Fei1, 2, 3, JIN Yanling1, 3, ZHAO Yun4, GAO Xiaofeng1, 2, 3, LI Yuhao1, 2, 3, CHEN Qian1, 2, 3 & ZHAO Hai1, 3**
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China )
(2University of Chinese Academy of Sciences, Beijing 100049, China)
(3Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China )
(4College of Life Sciences, Sichuan University, Chengdu 610064, China)
Abstract Raw Canna edulis Ker, one of the non-food crops, was utilized in this study to produce butanol by Clostridium
acetobutylicum 8012. posite Desi