文档介绍：苦参碱、氧化苦参碱、槐定碱对巨噬细胞活性及分泌肿瘤坏死因子-α的影响
作者:刘太华,刘德芳,王骏,汪晓军,罗陈
【摘要】目的观察苦参碱、-α(TNF-α)的影响。方法 ,不同浓度的苦参碱、 d,采用MTT法检测不同浓度的苦参碱、;收集培养液,采用ELISA方法检测每份培养液中TNF-α的含量。结果不同浓度的苦参碱、,不同浓度的苦参碱、-α有抑制作用,浓度越大,抑制作用越强,-α的抑制作用更明显。结论苦参碱、-α的分泌,苦参碱的抑制作用更明显。
【关键词】苦参碱;氧化苦参碱;槐定碱;巨噬细胞;肿瘤坏死因子-α;银屑病
Abstract:Objective To observe the effects of matrine, oxymatrine and sophordine on activity and TNF-α secretion of macrophage . Methods Macrophage were cultivated in 6 holes board and cultivated 1 day with different concentration matrine, oxymatrine and sophordine. Macrophage activity was measured with MTT and content of TNF-α in collected culture medium was measured with ELISA. Results Macrophage activity and the function of secreting TNF-α was restrained by matrine, oxymatrine and sophordine. The higher concentration of matrine, oxymatrine and sophordine was, the more intense the inhibiting effect was. The effect of matrine was more intense to the function of secreting TNF-α of macrophage. Conclusion Matrine, oxymatrine and sophordine can restrain macrophage activity and the function of secreting TNF-α, the effect of matrine is the most intense.
Key words:matrine;oxymatrine;sophordi