文档介绍：Continued clearance of apoptotic cells critically
depends on the phagocyte Ucp2 protein
Nature 477, 220–224
2011-9-30
Yan Xiaoxiang
Mitochondria: central platform for innate immunity
EMBOreports VOL12 | NO9 | 2011
Mitochondrial ROS and innate immune responses
Mitochondrial DAMPs
Mitochondrial involvement in cellular damage responses
Knockdown of UCP2 provide a ‘loss of function’ for
engulfing apoptosis cells
Positive control
Phagocytosis of apototic cells pany with enhanced mitochondrial membrane potential.
Engulfment of apoptotic cells leads to a modest increase in
intracellular ATP
10%
Indicating a mechanism for uncoupling nutrient oxidation from ATP generation
UCP2 mediate uncoupling the proton gradient and ATP
generation
• UCPs regulate the mitochondrial membrane potential through dissipation
of the proton gradient across the inner mitochondrial membrane, without
ATP generation.
• Compared to Ucp1 (restricted to brown adipose tissue), Ucp2 is more
ubiquitously expressed, with expression also occurring in several
phagocytic cell types.
Phagocytes engulfing apoptotic cells upregulate Ucp2 , and Ucp2 expression is
essential for phagocytic capacity.
Over-expression of UCP2 provide a ‘gain of function’ for
engulfing apoptosis cells