文档介绍：资料
山丹丹鳞茎多糖的提取及含量测定
摘要:山丹丹多糖是山丹丹主要的有效成分,从山丹丹粗多糖中分离纯化出纯化的山丹丹多糖,并对其含量进行测定。用水提醇沉法从植物山丹丹中提取百合粗多糖,再采用Sevage法去除蛋白。除蛋白后的沉淀物依次用无水乙醇、丙酮、无水乙醚各淋洗得到精制百合粗多糖。试验选取固液比、提取时间、提取温度3个因素,在单因素试验的基础上,采用L9 (33)正交试验设计,确定了最佳的提取条件为:提取温度70℃、固液比1:15、提取时间4h。选择此条件提取药用百合粗多糖,%。
关键词:山丹丹;多糖;提取;含量测定;正交试验
Bulb of Lilium concolor pellet extraction and content determination of haride
Abstract: Lilium concolor pellet haride is Lilium concolor pellet ponent, from Lilium concolor pellet in the crude harides purified purified Lilium concolor pellet haride, and determine the content of. Water extraction and alcohol precipitation from plant extraction of lily Lilium concolor pellet haride, removing protein by Sevage method. In addition to protein precipitate in ethanol, acetone, ethyl ether the eluent to obtain refined Lily haride. Test selection ratio of solid to liquid, extraction time, extraction temperature3 factors, based on the single factor experiment, by using L9(33) orthogonal experimental design, the optimum extraction conditions for the extraction temperature 70 degrees1: 15, the ratio of solid to liquid, extraction time4h. Select this condition extracting Lilium haride, extraction rate of %.
Key words: Lilium concolor pellet; haride; extraction; content determination; orthogonal test
山丹丹是百合科(ae)百合属(Lilium)的物种,是多年生草本植物,山丹丹学名斑百合,别名红百合,生活中经常被称为“山丹丹花”,百合科,多年生草本。地下具卵球形鳞茎,有少数白色肉质鳞片。茎直立,高30─60厘米,无