文档介绍：192 食品与药品 Food and Drug 2015年第17卷第3期
·技术交流·
HPLC法测定拉米夫定片的含量
董芳
(淄博市食品药品检验检测中心,山东淄博 255086)
摘要:目的建立高效液相色谱法(HPLC)测定拉米夫定片含量的方法。方法色谱柱为Agilent ZORBAX
SB-C1柱(250 mm× mm,5 μm),流动相为甲醇- mol/L的乙酸铵溶液(5:95),进样量10 μL,柱
温35 ℃,检测波长277 nm。~ μg/mL浓度范围内线性良好,r=;平均回收率
%。结论此方法结果准确、专属性强,可用于该药品的质量控制。
关键词:拉米夫定片;高效液相色谱法;含量测定
中图分类号:R917 文献标识码:A 文章编号:1672-979X(2015)03-0192-03
Determination of Lamivudine Tablets by HPLC
DONG Fang
(Zibo Food and Drug Inspection & Testing Center, Zibo 255086, China)
Abstract: Objective To establish an HPLC method for the determination of lamivudine tablets. Methods An Agilent
ZORBAX SB-C1 column(250 mm× mm, 5 μm)was adopted. The mobile phase was the mixture of methanol
and mol/L ammonium acetate solution (5:95). The injection volume was 10 μL. The column temperature was 35
℃ and the detection wavelength was 277 nm. Results This method had a good linearity in the range of -
μg/mL for lamivudine (r=). The average recovery was %. Conclusion The method is accurate, reliable and
specific, which can be used for the quality control of lamivudine tablets.
Key Words: lamivudine tablets; HPLC; determination
拉米夫定(lamivudine)是一种新型的核苷类抗式是对病毒DNA链的合成抑制,通过结合到新合成
病毒药,属合成的核苷类似物,本身没有活性,在人的HBV-DNA中来使DNA链的延长终止,从而达到
体被细胞激酶转化为活性代谢物三磷酸拉米夫定, 抑制病毒复制的作用[1-3]。英国Glaxo Operations (UK)
其作为HIV逆转录时的链终止物而选择