文档介绍：卡维地洛抗动脉粥样硬化作用的研究
【摘要】目的探讨卡维地洛在动脉粥样硬化病变中的抗氧化作用及其对增殖细胞核抗原(PCNA)及核因子kappaBp65(NFκBp65)活化的影响。方法雄性新西兰大耳白兔24只,随机分为正常组、高脂组、卡维地洛组,每组8只,共计喂养10周。实验结束后,酶法检测各组家兔的血清三酰甘油及胆固醇,黄嘌呤氧化法检测血清超氧化物歧化酶(SOD),硫代巴比妥酸比色法检测丙二醛(MDA),A及NFκBp65的表达。结果高脂组和卡维地洛组血清三酰甘油和胆固醇较正常组明显升高(F=、,q=~,P<),而该两组间差别无统计学意义(q=、,P>)。高脂组较正常组SOD明显降低,MDA明显增加,而卡维地洛组较高脂组SOD有所升高,MDA有所降低(F=、,q=~,P<)。A和NFκBp65的表达较正常组明显增加,A和NFκBp65的表达较高脂组明显减少(F=、,q=~,P<)。PCNA和NFκBp65的呈正相关(r=,P<)。结论卡维地洛有减轻脂质过氧化损伤的作用,可阻断NFκB的活化,进而抑制平滑肌细胞的增殖。
【关键词】动脉硬化卡维地洛抗氧化剂细胞增殖兔
[ABSTRACT]ObjectiveTo study the effect of carvedilol on antioxidation and activation of PCAN and NFκB on four male New Zealand white rabbits were randomly divided into three groups: normal group, high
fat diet group and cavediloltreated group, with eight rabbits in each group. All the rabbits were fed for 10 weeks. pletion of the experiment, the levels of serum triglyceride and cholesterol were measured by enzymatic method, of serum SOD by xanthine oxidative technique, and of serum MDA by thiobarbituric acid method. Expression of the PCAN and NFκBp65 in the vascular wall was detected immunohistochemically. pared with those of normal rabbits, the levels of serum triglyceride and cholesterol of the highfat diet and cavediloltreated were extremely high