文档介绍：自身免疫性肝炎患者血清中高迁移率族蛋白B1检测
作者:赵擎,辛绍杰,侯俊,胡燕,貌盼勇
【摘要】目的采用ELISA检测方法,初步探讨人高迁移率族蛋白B1(HMGB1)与自身免疫性肝炎(AIH)发病的相互作用关系。方法将纯化的HMGB1蛋白,包被ELISA检测微孔板,检测AIH患者和健康献血员的血清分别165份和69份,分析比较AIH患者和健康献血员的血清中抗HMGB1抗体的分泌情况。结果 AIH患者血清中抗HMGB1抗体的阳性率明显高于健康献血员(% VS % ,P<)。直线回归分析显示,抗HMGB1抗体滴度(OD值)与ALT水平有很好的相关性(相关系数r=)。结论 HMGB1是AIH疾病发生发展中一个重要的炎症蛋白,与疾病的炎症活动程度有很好的相关性。
【关键词】高迁移率族蛋白B1 自身免疫性肝炎 ELISA
Abstract: Objective Adaption the method of ELISA, investigation the relationship between the human high mobility group box-1 protein (HMGB1) and autoimmune hepatitis (AIH). Methods Coating the microwell plate using purified HMGB1 protein, detection the serum of the 165 AIH and 69 healthy respectively. Comparison the level of Anti-HMGB1 antibody in AIH and the healthy. Results The positive ratio of Anti-HMGB1 antibody in AIH more than that in healthy (% VS % ,P<), The linear regression analysis showed there were good correlation between the anti-HMGB1 antibody titer and the lev
el of alanine transarninase(ALT) (correlation coefficient, r=). Conclusion HMGB1 is a crucial inflammatory protein in the progressing of AIH, there are good correlation between the anti-HMGB1 antibody titer and the stage of inflammation in AIH.
Keywords:high mobility group box-1 protein (HMGB1);autoimmune hepatitis( AIH);ELISA
血循环中自身抗体的检测是自身免疫性肝炎(AIH)诊断和分型的主要依据。但自身抗体的检测并不能完全排除其他原因引起的肝脏病变,药物性肝炎、病毒感染等