文档介绍：结核分枝杆菌分泌蛋白Ag85B的免疫学特性
作者:薛莹李元史皆然师长宏李别虎范雄林柏银兰马文煜
【关键词】分枝杆菌
关键词: 分枝杆菌;Ag85B蛋白;免疫学
摘要:目的研究原核表达的结核分枝杆菌分泌蛋白Ag85B的免疫学特性. 方法用原核表达的Ag85B蛋白免疫BALB/c小鼠,,体外用抗原再刺激,MTT方法检测IFN-γ产生水平和MTT法检测淋巴细胞增殖. 结果 Ag85B免疫小鼠可引起较高水平的特异性抗体应答,免疫小鼠的脾淋巴细胞体外用抗原再刺激,-γ量为960pg・L-1 ,而生理盐水对照组的量低于80pg・L-1 . 结论 Ag85B有可能作为新型结核疫苗的组分.
Keywords:mycobacterium;Ag85B;immunobiology
Abstract:AIM To investigate the immunobiological proper-ties of secretory protein Ag85B of MTB BALB/c mice were immunized with was used to detect the anti-Ag85B antibody titer in the immunized mice of IFN-γsecreted from splenocytes of the
immunized mice after specific-antigen stimulation was detect-ed by using a cytopathic effect reduction assay and splenocytic proliferation of the immunized mice in response to antigen restimulation was detected by MTT Ag85B-immunized mice induced higher anti-Ag85B antibody titer,and protective cellular-mediated immunity was charac-terized by splenocytes which upon antigen-specfic stimulation secreted increased levels of IFN-γand proliferated significant┐ Ag85B can be used as a ponent of the new TB ine.
0 引言
,由于卡介苗(BCG)保护性的不完善,结核菌耐药菌的产生,爱滋病的流行,世界人口的剧增,全球性旅游业的发展以及吸毒、酗酒、贫穷等原因,结核病呈日益严重的回升趋势,再次成为危害人类健康的世界性疾病[1] .因而,积极研究结核病