电针对血管性痴呆大鼠学习记忆能力的改善作用及其机理探讨.doc

文档介绍：电针对血管性痴呆大鼠学忆能力的改善作用及其机理探讨
作者:闫兵,许能贵,唐纯志,何利雷,绍瑛
【摘要】【目的】观察电针对血管性痴呆(vascular dementia,VD)大鼠学忆能力的影响及其可能的机理。【方法】选择SPF级成年健康SD大鼠50只,随机抽取8只大鼠为假手术组,其他42只均采用4血管阻断法复制缺血模型。将造模成功的大鼠按随机数字表法分为电针组、尼莫通组和模型组。假手术组和模型组同等条件下饲养,未予任何治疗。电针组针刺模型大鼠头部百会穴,背部双侧膈俞穴、脾俞穴和肾俞穴,连接电针仪,施以连续波,频率150?Hz,1次/d,连续治疗15?d。尼莫通组模型大鼠给予尼莫通12?mg/kg,1次/d,连续15?d。治疗15?d后采用Morris水迷宫学忆能力,采用双波长分光光度计法检测大鼠血浆CO含量,分光光度比色法测定缺血脑组织中NO的含量。【结果】模型大鼠表现出明显的学忆障碍,在水迷宫实验中,其逃避潜伏期显著性延长,在原平台象限跨越相应平台次数与其他3个象限无显著性差异,血浆CO和脑组织NO水平显著性升高。而电针组逃避潜伏期较模型组显著性缩短,相同时间内跨越原平台次数显著性多于其他3个象限,血浆CO和脑组织NO含量较模型组大鼠显著降低(均P<005或P<001)。【结论】电针治疗血管性痴呆的作用可能与其改善VD大鼠学忆能力,显著性降低血浆CO和脑组织NO含量有关。
【关键词】痴呆,血管性/针刺疗法;疾病模型,动物;穴,百会;穴,膈俞;穴,脾俞;穴,肾俞;大鼠

【Methods】Fifty specific pathogen free(SPF) adult rats were randomized into four groups. Except the 8 rats in the pseudooperation group (group A), the other 42 rats were induced cerebral ischemia by fourvessel occlusion the model rats were randomized into model group (group B), EA group (group C) and nimotop group (group D). Groups A and B were fed with the same forage and received no medication. Group C received EA on the acupoints of Baihui, Geshu, Pishu and Shenshu, and EA was set in continuous wave with the frequency being 150Hz, once per day and lasting 15 consecutive days. Group D received nimotop 12mg/kg, qd, for 15 consecutive days. After 15day treatment, the learning and memory abilities