文档介绍：不同处理对大鼠移植心脏存活时间的影响
【摘要】目的观察供体脾细胞(SPC)与环磷酰胺(CP)联合预处理对大鼠移植心脏存活时间的影响。方法应用大鼠颈部心脏移植模型,实验分为对照组(未干预组)、CsA组、SPC+CP组。CsA组心脏移植后予环孢霉素A( CsA)10 mg/kg,SPC+CP组采用供体SPC和CP预处理移植受体,然后行大鼠颈部心脏移植术。观察移植心脏存活情况及组织病理变化。结果对照组、CsA组、SPC+CP组移植心脏存活时间分别为(±)、(±)、(±)天;组织病理检查显示,经SPC+PC预处理的移植心脏排斥反应明显减弱。结论 SPC 和CP 预处理可以诱导受体对移植物的免疫耐受,延长移植心脏的存活期,效果优于CsA。
【关键词】心脏移植免疫耐受供体脾细胞环磷酰胺环孢霉素A 大鼠
Abstract: Objective To evaluate the effect of pretreatment with donor spleen cells (SPC) and cyclophosphamide (CP) on the survival time of cardiac allograft. Methods The experiment was performed on SD rat models of heterotopic cervical heart transplantation. The animals were divided into 3 groups. The control group consisted of SD models without treatment. In the cyclosporine A (CsA) drug control group, the recipients were treated after transplantation with CsA 10 mg/kg ip q2d. In the SPC + CP pretreatment group, the SD rats were pretreated with SPC iv on D0 and with CP 80 mg/kg ip on D2, and the transplantation was carried out on D10. The living condition and the survival time of the allograft, and its pathology were noticed. Results The survival time in the control group, CsA group and SPC +CP group was ( ± ), ( ± ) and ( ± ) respectively. Histopathology showed the HVG rejection reactions were evidently attenuated in the pretreatment test group. Conclusion Pretreatment with SPC plus CP