文档介绍：摘要第一部分大鼠局灶性脑缺血后处理动物模型的建立关键词:脑缺血;缺血后处理;细胞凋亡目的:建立大鼠局灶性脑缺血后处理,模型,并确定对局灶性脑缺血再灌注琁/损伤发挥神经保护的最佳时间窗。方法:将成年健康雄性大鼠只,随机分为偈质踝椤⑷毖T俟嘧组/组、一椤组、一楹虸组。采用线栓法阻塞大脑中动脉琈建立局灶性脑缺血再灌注模型。口方法为在后对每个亚组大鼠分别给与鲅返////再灌注/缺血。所有动物于再灌注猩窬δ苋彼鹌婪郑⒉舛怨K捞寤细胞凋亡的变化。结果:用线栓法成功建立大鼠缺血再灌注模型。与组相比,.椤组、组大鼠神经功能缺损评分明显降低,.。组脑梗死体积及凋亡细胞计数显著增加,.组、.:改良的线栓法效果可靠,是制备再灌注的理想模型;缺血后处理能减轻局灶性的脑缺血再灌注损伤,其最佳时间窗为反复危/俟嘧ⅲ毖!
:探讨缺血后处理对大鼠局灶性脑缺血再灌注时样受体秃艘子表达的影响,阐明甂藕磐吩谀匀毖:蟠碇械作用。方法:将成年健康雄性大鼠只,随机分为假手术组、缺血再灌注/组腿毖:蟠碜:罅阶楦菰俟嘧⑹奔涿孔橛址治、、甯鲅亲2捎孟咚ǚㄗ枞竽灾卸⒕灶脑缺血模型,后处理方法为大脑中动脉阻闭后持续再灌注前,行鲅返再灌注/缺血。。对各组进行神经功能缺损评分,旧ú。结果:楹涂谧榇笫缶杉窬δ苋彼鸺叭毖2啻竽园肭蚬K馈S隖橄比,榇笫竽怨K捞寤飨约跎,神经功能缺失评分明显改善/門鞍准癿谠俟嘧J忌撸达高峰,之后逐渐下降。口组蛋白及表达时间曲线与组相一致,但与组各相应亚组比较,榈腡鞍准癿泶锞飨越档唾。甂鞍准癿贔楹虸榫朐俟嘧撸达高峰后下降,与时间变化曲线相~致。与组各相应亚组比较,:毖T俟嘧⒖梢餞蚇狵谋泶铩能抑制缺血再灌注后谋泶铮跣∪毖T俟嘧⑺鹕耍纳粕窬形9δ堋。和..
关键词脑缺血;再灌注;缺血后处理;样受体缓艘蜃觢【赵仁亮教授硕士研究生王鹏窬⊙指导教师
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目录第一部分大鼠局灶性脑缺血后处理动物模型的建立前言································································ぁ实验材料和方法·······························ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁ结果··································································讨论··································································小结·························ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁ参考文献······························································第二部分缺血后处理对大鼠局灶性脑缺血再灌注时和稫泶锏挠跋前言·······