文档介绍：河北大学
硕士学位论文
麻黄碱分子印迹聚合物的制备及其应用
姓名:田硕
申请学位级别:硕士
专业:分析化学
指导教师:郭志峰
2011-06
摘要
摘要
分子印迹技术(Molecular Imprinting Technique, MIT)是一种可以制备对于给定化合
物具有特定识别性的聚合物的技术,这种技术正在快速发展。由于对模板分子具有特异
选择性,制备的分子印迹聚合物(MIP)能够从复杂样品中选择性提取目标分子,其作
为固相萃取的吸附材料越来越受到关注。
本文采用水溶性共聚物为功能单体,麻黄碱为模板分子,制备出了带有麻黄碱分子
印迹的共聚物树脂。以制备的分子印迹聚合物为吸附剂,对麻黄碱和它的手性对称体伪
麻黄碱,做了静态平衡吸附与固相吸附实验。洗脱溶剂采用了三种不同极性试剂:二氯
甲烷、丙酮、水。
实验数据表明,印迹聚合物内新增了与麻黄碱分子空间位形一致的空穴,它对麻黄
碱具有吸附作用。吸附的样品分子麻黄碱,都能被 3 种洗脱剂有效洗脱出。丙酮洗脱效
果最佳,水次之,氯仿紧随其后。由此判断出,印迹空穴与样品分子的作用,为非共价
键,属物理吸附。
印迹树脂对麻黄碱以及质子化麻黄碱均有吸附作用,同时非印迹树脂对麻黄碱也有
吸附作用。于是我们用伪麻黄碱做了对照试验,我们发现,麻黄碱印迹树脂对麻黄碱的
吸附率高达 %,而对伪麻黄碱的吸附率只有 %,这就进一步证实了印迹树脂
中麻黄碱分子印迹空穴的存在。印迹树脂的这个特性,可以被用来麻黄碱与伪麻黄碱的
手性分离。
应用实验是以制备的印迹树脂作固相吸附材料,对麻黄提取液中的麻黄碱,进行固
相吸附萃取。萃取结果与常规的液液萃取作了比较。结果表明,印迹树脂对麻黄碱具有
选择吸附性,固相萃出的麻黄碱是液液萃取的倍。

关键词分子印迹聚合物固相萃取麻黄碱伪麻黄碱

I
Abstract
Abstract
Molecular imprinting technology(MIT)is a rapidly developing technique for the
preparation of polymers having specific molecular recognition properties for a given
compound. With recognition ability for the template molecule, molecularly imprinted polymer
(MIP) is able to selectively extract analyte plex samples and are growing of interest
for their potential applications as advanced materials for solid-phase extraction.
In this paper, A molecularly imprinted co-polymer(MIP) with special molecule
recognition properties of ephedrine was prepared by self-made water-solubility resin and
ephedrine as template molecule. The self-made resin as the adsorbent, We done Equilibrium
binding adsorption and solid-phase extraction to the ephedrine and its chiral symmetric.
Elution solvent polarity using three different reagents: chloroform, acetone, water.
The results showed that:in the copolymer resin,there were the new cavitis which had the
same spatial configuration with ephedrine molecule, and ephedrine could be adsorbed by the
new cavitis. The sample molecules ephedrine which were adsorbed could be effectively