文档介绍：DNA修复酶在胃癌组织中的表达变化及临床意义
(作者:___________单位: ___________邮编: ___________)
作者:吴迎爽周宁程毅魏贵亮谢震祁贺薄爱华张晓丽邢立强
【摘要】目的:检测胃癌组织中DNA损伤修复酶(O6—甲基鸟嘌呤—DNA甲基转移酶O6—methylguanine DNA methyltransferase, MGMT)的表达变化,探讨其表达和胃癌临床病理特征之间的关系,进一步用于指导临床化疗方案的制定。方法:采用免疫组化方法(SABC法)检测55例胃癌标本中MGMT的表达情况。结果:%(24/55),其阳性表达与年龄、病理类型有关,而与性别`、分化程度、侵犯深度、淋巴结转移的相关性不明显。结论:MGMT对胃癌化学治疗方案的制定有指导作用。
【关键词】胃癌;DNA损伤修复酶;免疫组化
【ABSTRACT】 Objective: To investigate the expression change of DNA repairing enzyme MGMT in gastric carcinoma,and the relationship between the expression and pathologic charateristic,so as to guide makingup of treating plan. Methods: Detect the expression of 55 cases with gastric carcinoma by SABC immunohisto
chemistry. Results:The positive expression rate of MGMT in the gastric carcinoma was %(24/55).There was a signficant statistic difference between age and pathologic type in the expression of MGMT, but no obvious correlation among sex, defferentiation degree,invasion depth and lymphnode :The expression of MGMT can guide to make up a plan for treating gastric carcinoma.
【KEY WORD】 Gastri carcinoma; DNA repairing enzyme(MGMT); Immunohistochemistry
烷化剂与DNA相互作用可以在DNA分子中形成各种烷化碱基,其中O6—甲基鸟嘌呤与基因突变和癌症发生关系最为密切。生物体内含有一种高度专一性DNA修复酶即O6—甲基鸟嘌呤—DNA甲基转移酶(O6—methylguanineDNA methyltransferase MGMT),该酶能够有效地修复DNA分子中O6位鸟嘌呤的烷化碱基,在防止烷化剂诱导的致癌、致突变和抗癌化疗过程中均起重要作用[1],从而有效维持着基因组的稳