文档介绍：H2S对兔离体心脏心率的影响
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作者:陈立锋徐涛范凌赵兰平陈少峰温晓竞张晓云马建伟尹万斌
【摘要】目的:探讨硫化氢(H2S)对兔离体心脏心率的影响。方法:用标准微电极细胞内记录技术,观测硫化氢对兔右心房心肌细胞动作电位频率的影响。观测指标:自发放电频率(rate of pacemaker firing,RPF)。结果:(1) 用200μmol/LNaHS 灌流时,和正常对照组相比,RPF显著减小()。(2) 400μmol/L NaHS与对照组比较, RPF显著减小()。和200μmol/LNaHS相比,RPF显著减小()。(3)以上使RPF减小的效应在NaHS灌流3~5min出现,10min时已经有显著减小(),25~30min达到高峰。结论:H2S可浓度依赖性的影响兔离体心脏的心率。
【关键词】心率;电生理; 硫化氢;心脏;兔
Hydrogen sulfide(H2S)has been proved to be the third endogenous signaling gasotransmitter,besides nitric oxide(NO) and carbon monoxide(CO).It shows important physiological functions,involving in the longterm potentiation in the hippocampus in the central nervous system,relaxing gastrointestinal trac
t smooth muscles[1,2].H2S has been also found to play many regulating functions in cardiovascular system,and play a certain effect in many cardiovascular diseases[3].But the effect on heart rate hasnt been deeply using standard intracellular microelectrode technique to record Aps,hydrogen sulfide was used to investigate the rate of pacemaker firing of left aim of the study was to observe the effects of hydrogen sulfide on heart rate of rabbit hearts in vitro,elucidate the mechanism(s) involved,provide electrophysiologic proof for the nosogenesis of some cardiovascular diseases and provide research directions for the devel