文档介绍：HIV1儿童感染者CD3dimCD56+细胞数量及其受体变化的研究
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作者:傅更锋郝莎赵俊丽徐晓琴丁萍还锡萍羊海涛侯亚义
【摘要】目的:研究人类免疫缺陷病毒1(HIV1)儿童感染者体内CD3dimCD56+细胞数量及部分细胞受体的变化。方法:采集11例经确认的HIV1儿童感染者和10例HIV1抗体阴性的健康儿童抗凝全血,应用流式细胞术对CD3dimCD56+细胞的数量和细胞上部分受体的表达进行测定。结果:HIV1儿童感染者CD3dimCD56+细胞的数量显著多于健康儿童(P<);HIV1儿童感染者与健康儿童比较,CD16无变化(P>),NKp30、NKG2D、KIR2DL1和CD244的表达极显著升高(P<),NKp44、NKp46、KIR3DL1和NTBA的表达显著升高(P<),NKp80的表达极显著降低(P<)。结论:HIV1儿童感染者CD3dimCD56+细胞的数量显著多于健康儿童,并且细胞上受体的表达发生剧烈变化。
【关键词】人类免疫缺陷病毒1 儿童感染者 CD3dimCD56+细胞受体

Abstract:Objective To study the changes of CD3dimCD56+ cell and its receptors in HIV
1 infected children. Methods Blood samples from 11 HIV1 infected children and 10 healthy children were collected in EDTA coated number of CD3dimCD56+ cells and the expression of its receptors were determined by flow cytometry. Results The number of CD3dimCD56+ cell in HIV1 infected children was higher than that in healthy children. Compared with healthy children,no change was found in the expression of CD16 receptor in HIV1 infected children,but the expression of NKp30,NKG2D,KIR2DL1 and CD244 receptors was significantly increased(P<).The expression of NKp44,NKp46,KIR3DL1 and NTBA in HIV1 infected children was also increased(P<),while the expression NKp80 receptor was decreased obviously(P<). Conclusion The number of CD3dimCD56+ cell in HIV1 infected children was higher than that in healthy expression of CD3dimCD56+ receptors changes dramatically in HIV1 infected children.

Key words:human immunodeficiency virus1; infected children; CD3dimCD56+ cell; receptor
CD3dimCD56+细胞是一种介于NK细胞和T细胞之间的特殊细胞群,在人体对外界病原微生物侵袭的免疫调节中发挥着重要作用。CD3dimCD56+细胞主要为NKT细胞,NKT细胞在人体的含量很低,%~1%[1]。在CD3dimCD56+细胞的表面表达有NK细胞受体和部分T细胞受体,通过这些细胞受体的变化,CD3dimCD56+细胞可以调节NK细胞、巨噬细胞、CD4+和CD8+ T细胞等效应细胞表达分泌Th1和Th2等细胞因子[2]。
CD3dimCD56+细胞主要为NKT细胞,NKT细胞表达CD4的同时高表达细胞趋化因子受体
R5),因而CD3dimCD56+细胞也是人类免疫缺陷病毒1(HIV1)感染的靶点之一,所以CD3dimCD5