文档介绍：HPLCELSD法测定桔梗中3种桔梗皂苷的含量
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作者:叶静,肖美添,汤须崇,黄雅燕
【摘要】目的建立测定桔梗药材中桔梗皂苷D、D3、E含量的方法。方法采用高效液相色谱蒸发光激光闪射检测器(HPLCELSD)法,Hypersil C18柱(5μm,.6mm×150mm),以乙腈-水为流动相梯度洗脱,,ELSD漂移管温度113℃,载气(N2)。结果桔梗皂苷D、D3、~(r=)、~(r=)、~(r=),平均回收率(n=5)%、%%。结论本法操作简便,结果准确可靠,可用于桔梗皂苷D、D3、E含量的测定。
【关键词】桔梗;桔梗皂苷;HPLCELSD
ABSTRACT: Objective To establish a quantitative method to determine the content of platycodin D, D3 and E in Radix High performance liquid chromatagraphyevaprorative light scattering dector (HPLC
ELSD) method was adopted. Platycodin D, D3 and E were separated by C18 (5μm, ×150mm) with acetonitrilewater as mobile phase in a gradient program; flow rate was , the temperature of drift tube was set at 113℃, and the gas flow (N2) was set at The linear ranges of platycodin D, D3 and E were - (r=), - (r=) and - (r=), respectively. The average recoveries (n=5) were %, % and %, The method is convenient, accurate and reliable, and can be used for the determination of platycodin D, D3 and E in Radix platycodoi.
KEY WORDS: Radix platycodoi; platycodin; HPLCELSD
中药桔梗(Radix platycodi)为桔梗科植物桔梗Platycodon grandi