文档介绍：IL1β、幽门螺杆菌cagA与人慢性胃炎的关系
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作者:乔文,李娜,李长顺,薛挥
【摘要】目的探讨IL1β、幽门螺杆菌cagA基因(cytotoxin A)和人慢性胃炎之间的相关性。方法收集西安交通大学医学院第一附属医院胃镜检查的胃活检标本,经甲醛固定,制备石蜡切片,进行病理分型和IL1β SABC免疫组化染色;同时作幽门螺杆菌培养,16s rRNA PCR鉴定,检测cagA基因。结果慢性萎缩性胃炎组IL1β表达的阳性率高于慢性浅表性胃炎组();慢性萎缩性胃炎组中,异型增生组IL1β表达的阳性率高于肠腺化生组()。幽门螺杆菌cagA基因阳性率在慢性萎缩性胃炎组和慢性浅表性胃炎组无显著性差异(),异型增生组cagA基因阳性率和肠腺化生组亦无显著性差异()。结论 IL1β在慢性胃炎的转归和发展中具有比cagA基因更高的预测价值。
【关键词】 IL1β;幽门螺杆菌;cagA基因;慢性胃炎
ABSTRACT: Objective To discuss the correlation between IL1β, cagA of Helicobacter Pylori and chronic gastritis. Methods We collected the gastric biopsy specimens by gastroscopy for pathologic examination and culture of Helicobacter pylori. In the meantime, IL
1β was detected by SABC histoimmunology and cagA gene was tested by polymerase chain reaction. Results The positive rate of IL1β in chronic atrophic gastritis group was higher than that in chronic superficial gastritis group (). In the chronic atrophic gastritis group, the positive rate of IL1β in dysplasia group was higher than that in intestinal mesplasia group (). There was no obvious difference in the positive rate of cagA gene between chronic atrophic gastritis group and chronic superficial gastritis (). There was no obvious difference in the positive rate of IL1β between dysplasia group and intestinal mesplasia group (). Co